The amaR 2X PCR Mix is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. The amaR 2X PCR Mix is a pre-mixed solution containing Taq DNA polymerase, PCR reaction buffers, dNTPs, and gel loading dye. The amaR OnePCR which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3 ‘ → 5’ exonuclease activity. The amaR 2X PCR Mix can amplify DNA fragments up to 5 kb and with good amplification specificity, and it is compatible with the template. The PCR product has an A base at the 3′ end and c an be directly used for T/ A cloning after purification. The amaR OnePCR contents red tracking dyes that run at 10 bp on a 1% agarose gel. The amaR 2X PCR Mix mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 20 μl each.