The GScript First-Strand Synthesis Kit is a recombinant M-MLV RTase expressed in E. coli and purified to homogeneity. It has a lower RNase H activity and a high thermal stability. The enzyme is widely used to synthesize first-strand cDNA at temperatures up to 55°C with increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. It can generate cDNA from 100 bp to 12 Kb. The GScript RTase has a special activity with its secondary structure, rendering stabilization during the reverse transcription reaction.
|5X 1st strand buffer|