OnePCR™ HotStar

Cat. No.: SM206-0100
Size: 100 Reactions (2 X 1.25 ml)
Store at RT up to 1 month.
Store at 4°C up to 6 month.
Store at -20°C up to 1 year.
Shipping Temperature: 4°C
Note: OnePCR™ HotStar is light sensitive and should be stored and protected from light.

Features

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No destaining requirement.
  • Compatibility – Use the Blue Light or UV to detect the signal.
  • Specificity – Reduce the non-specific amplification products, thus
  • significantly improving the specificity of PCR reactions.

描述

OnePCR™ HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ HotStar is a pre-mixed solution containing Hot start Taq DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. OnePCR™ HotStar, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. Hot start Taq DNA polymerase has a nontemplate– dependent terminal transferase activity that adds a 3’
deoxyadenosine to product ends, and has a 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). OnePCR™ HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. The enzyme s contains a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the initial denaturation step in PCR cycling at 94°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR.

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Protocol
MSDS