OnePCR™ HotStar is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ HotStar is a pre-mixed solution containing Hot start Taq DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. OnePCR™ HotStar, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. Hot start Taq DNA polymerase has a nontemplate– dependent terminal transferase activity that adds a 3’
deoxyadenosine to product ends, and has a 5’→3’ exonuclease activity (but not 3’→5’ exonuclease activity). OnePCR™ HotStar mixture is supplied at the 2X concentration to allow 50% of the final reaction volume to be used for the addition of primer and template solutions. The enzyme s contains a proprietary antibody that blocks polymerase activity at ambient temperatures. Activity is restored after the initial denaturation step in PCR cycling at 94°C, thereby providing an automatic “hot start” for Taq DNA polymerase in PCR.