PCR SuperMix provides qualified reagents for the amplification of nucleic acid templates by the polymerase chain reaction (PCR). PCR SuperMix contains Mg++, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient to allow
amplification during PCR. PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 μl
each are provided.
PCR SuperMix may be stored at either -20°C or 4°C. Storage at 4°C avoids the necessity of thawing the mix before assembling the PCR. No detectable reduction of PCR performance or enzyme activity is observed after storage of PCR SuperMix for 12 months at 4°C. Repeated freeze-thaw cycles do not reduce performance or activity.