OnePCR™

Cat. No.: SM203-0100
Size: 100 Reactions (2 X 1.25 ml)
Store at RT up to 3 month.
Store at 4°C up to 6 month.
Store at -20°C up to 1 year.
Shipping Temperature: 4°C
Note: OnePCR™ is light sensitive and should be stored and protected from light.

Features

  • No post-staining processing of DNA required.
  • No need to prepare PCR Reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity – High degree of sensitivity as the ethium bromide.
  • Speed – No destaining requirement.
  • Compatibility – Use the blue light or UV to detect the signal.

描述

OnePCR™ is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ is a pre-mixed solution containing Taq DNA polymerase, PCR Buf fer, dNTP, gel loading dyes, and fluorescence dye. OnePCR™ which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity . OnePCR™, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR™ mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.

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