ITS-M
Why does growth rate (doubling time) become slowly when addingITS-M?
Stock ITS-M is 500X and working concentration is 1X. Please check working concentration of ITS-M first.
If working concentration of ITS-M is correct. Slow growth might due to adaption of new culture supplement, especially for adhesion cell line. According to our experiments, it might take 2 weeks to come back to normal doubling time.
In addition, we suggest to adjust ratio of serum and ITS-M progressively (gradually reduce % of FBS) for cells adapting to new growth environment.
Stock ITS-M is 500X and working concentration is 1X. Please check working concentration of ITS-M first.
If working concentration of ITS-M is correct. Slow growth might due to adaption of new culture supplement, especially for adhesion cell line. According to our experiments, it might take 2 weeks to come back to normal doubling time.
In addition, we suggest to adjust ratio of serum and ITS-M progressively (gradually reduce % of FBS) for cells adapting to new growth environment.
Why does some precipitation observed after storing at 4°C?
Due to cholesterol will be precipitated after storing at 4°C.
We suggest to centrifuge tube by 12,000g for 3 minutes and aliquot from supernatant.
Due to cholesterol will be precipitated after storing at 4°C.
We suggest to centrifuge tube by 12,000g for 3 minutes and aliquot from supernatant.