NAP-Genomic DNA
Why was the genomic DNA recovery quantity low
Buffer B with the incorrect ratio was added to the amplification reaction.
Verify that an equal volume of the Buffer B was added to the reaction.
The ethanol(96~100%) was not added to the W2 buffer.
The gel slice did not dissolve completely.
The gel slice was too big. If the column was overloaded, decrease the loading volume. If overloaded , separate into 2 columns. If the DNA fragments were more than 300mg, separate the gel slice into two microcentrifuge tubes.
Dissolved incompletely: Increase time for the Gel Extraction Step until the gel slice has completely dissolved. Use an equal volume of the Buffer B and/ or low-melting-point agarose gels.Incorrect elution conditions: Ensure that the Buffer E or ddH2O is added into the center of the PG Column.
The recovery buffer volume was too small. Increase the amount of the Buffer E to at least 50 μl for use.
Buffer B with the incorrect ratio was added to the amplification reaction.
Verify that an equal volume of the Buffer B was added to the reaction.
The ethanol(96~100%) was not added to the W2 buffer.
The gel slice did not dissolve completely.
The gel slice was too big. If the column was overloaded, decrease the loading volume. If overloaded , separate into 2 columns. If the DNA fragments were more than 300mg, separate the gel slice into two microcentrifuge tubes.
Dissolved incompletely: Increase time for the Gel Extraction Step until the gel slice has completely dissolved. Use an equal volume of the Buffer B and/ or low-melting-point agarose gels.Incorrect elution conditions: Ensure that the Buffer E or ddH2O is added into the center of the PG Column.
The recovery buffer volume was too small. Increase the amount of the Buffer E to at least 50 μl for use.
Why was the RNA contamination present?
Please use RNase A to remove RNA.
Please use RNase A to remove RNA.
Why was the Inhibition of downstream enzymatic reactions present?
It indicated the residual presence of alcohol. Place the column in the oven at 60°C for 10 minutes.
It indicated the residual presence of alcohol. Place the column in the oven at 60°C for 10 minutes.
Why was the column clogged?
The sample was added excessively, causing the lysis step to be executed incompletely. When sucking the supernatant, avoid taking in the impurities.
The sample was added excessively, causing the lysis step to be executed incompletely. When sucking the supernatant, avoid taking in the impurities.