Ultrafect™ Reagent System

Cat. No. SM502-1000

Size: 1 mL

Ultrafect carries most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results. It delivers superior transfection efficiency and improved cell viability. TransPlus Reagent includes in Ultrafect™ Reagent System could help DNA fragment folding and less degradation to improve the transfection efficiency.

描述

Features

  • With transfection-enhancer increase effective DNA transfection
  • Lower cytotoxicity

Performance test data

  • Cell: H1299
  • Sample type: Plasmid DNA, GFP
  • Detection: Microscope & flowcytometry

 

Note

  • Do not add the antibiotics to the medium during transfection as this causes cell death.
  • Maintain the same seeding conditions between experiments.
  • For most cell lines, transfection in the absence of serum is optimal. For HeLa cells, high-level transfection requires the presence of serum in the medium during transfection. This option should be tested if optimization does not yield high-level transfection.
  • Some no serum medium might inhibit the transfection of cation ion and plasmid. We recommend using the reduced serum medium without serum.
  • Different cell types and number of passages might lead to different transfection efficiency, and we recommend using at least two different concentrations of transfection reagent as the control in new transfect experiments to optimize experimental conditions.
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ProtocolSDS
SM502-1000A_SDS
SM502-1000B_SDS