OnePCR™ HiFi

Cat. No.: SM205-0100
Size: 100 Reactions (2 X 1.25 ml)
Store at RT up to 1 month.
Store at 4°C up to 6 month.
Store at -20°C up to 1 year.
Shipping Temperature: 4°C
Note: OnePCR™ HiFi is light sensitive and should be stored and protected from light.

Features

  • No post-staining procession.
  • No need to prepare PCR reagents.
  • Direct loading onto your agarose gel for analysis.
  • Sensitivity- High degree of sensitivity as the ethium bromide.
  • Time Efficiency- No destaining requirement.
  • Compatibility – Use the Blue Light or UV to detect the signal.
  • Effective for the amplification of GC-rich targets.
  • Exhibits strong 3’→5′ exonuclease activity.

描述

OnePCR™ HiFi is a ready-to-use PCR reaction mixture. Simply adding primers and template, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ HiFi is a pre-mixed solution containing GDP-HiFi DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. It contains the fluorescence dye, which is directly detected on the blue-light transilluminator or UV epi-illuminator after the DNA electrophoresis. The OnePCR™ HiFi mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the
addition of the primer and template solutions. Reagents are provided with sufficient amplification reactions of 50 μl each. OnePCR™ HiFi exhibits strong proof-reading activity. The GDP-HiFi DNA polymerase exhibits excellent processivity and elongation capability. The elongation rate of this enzyme is approximately 2 times higher than that o f Taq DNA polymerase. OnePCR™ HiFi has an extension rate of 106 to 138 nucleotides per second. OnePCR™ HiFi produces blunt end PCR products.

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Protocol
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