OnePCR™ HiFi is a ready-to-use PCR reaction mixture. Simply adding primers and template, the reagent will execute primer extensions and other molecular biology applications. OnePCR™ HiFi is a pre-mixed solution containing GDP-HiFi DNA polymerase, PCR buffer, dNTPs, gel loading dyes, enhancer, and fluorescence dye. It contains the fluorescence dye, which is directly detected on the blue-light transilluminator or UV epi-illuminator after the DNA electrophoresis. The OnePCR™ HiFi mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the
addition of the primer and template solutions. Reagents are provided with sufficient amplification reactions of 50 μl each. OnePCR™ HiFi exhibits strong proof-reading activity. The GDP-HiFi DNA polymerase exhibits excellent processivity and elongation capability. The elongation rate of this enzyme is approximately 2 times higher than that o f Taq DNA polymerase. OnePCR™ HiFi has an extension rate of 106 to 138 nucleotides per second. OnePCR™ HiFi produces blunt end PCR products.