Nimble Juice is a fast and sensitive fluorescent dye for visualization and quantitation of proteins separated by 1-D or 2-D SDS-PAGE. It comes as a 100x stock solution that is simply diluted with water by the user to its working concentration. Nimble Juice is normally low fluorescent but emits strong fluorescence (bright golden color) as bound to proteins. The staining procedure is a simple two-step protocol (fix and stain) that can be completed in as little as 30 minutes. Gels to be stained are fixed with ethanol/acetic acid solution prior to staining with Nimble Juice solution. A destain step is not normally recommended, but may be employed to reduce background, simply by agitating the gel in water for 1-5 minutes. Gels stained with Nimble Juice fluorescent gel stain may be directly visualized with a variety of different UV-based fluorescence imaging systems. The maximum emission wavelength of protein-bound Nimble Juice is near 570 nm. Nimble Juice gives exceptional sensitivity and wide dynamic range for protein detection. The bound Nimble Juice dye is easily removed from the protein by immersing the gel in sufficient water, thus it is well compatible with subsequent enzymatic digestion and mass spectrometry for proteomics applications. Stained gels may be stored in stain solution in the dark at 2-8°C; imaging sensitivity might be moderately enhanced after 4°C storage of the stained gel.
Equipment Required but Not Supplied
1. Staining containers—Glass trays are recommended.
2. Imaging equipment — Gels are best imaged using a UV-based fluorescence imager capable of excitation near 330 nm and 390 nm and detection near 570 nm.
3. Laboratory shaker or rocker.
4. Powder-free latex, vinyl, or nitrile gloves.
The product is stable for at least 6 months when stored at 2-8°C. Avoid exposure to temperatures greater than 37°C and protect from light.