ITS-M, 500X

Catalog No.: CC002-1000

Size: 1 ml

The ITS supplement was reported a substantial reduction in the Fetal Bovine Serum (FBS) requirement for routine maintenance. ITS-M is a mixture of insulin, transferrin, and sodium selenite which designed for use in general basal media such as DMEM, RPMI-1640 and nutrient media such as Ham’s F-12 or DMEM/F12.
When ITS-M is used with a low percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
It enhances cell growing under serum-reduced conditions and could decrease the serum requirement of a variety of cell types.

貨號: CC002-1000 分類:

描述

Specification

Size: 1 ml

Storage: 4°C

Working concentration

Per vial of ITS-M is sufficient for 500 ml of medium. In general, for optimal cell growth, it is necessary to add 1% serum for suspension cells; some adherent cultures may require 2% serum supplementation.

Formulation

ComponentConcentration (g/L)
Insulin5.00
Transferrin2.75
Sodium Selenite0.00335
Sodium Pyruvate
Ethanolamine
*Prepared in EBSS without Phenol Red.
Applications
Supplement of cell culture.
Caution
Research use only. Not for any animal or human therapeutic or diagnostic uses.

 


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